2024, Volume 70(74), Issue 3 (September), pp. 23 - 33

Phytochemical Screening of Sida Rhombifolia Knitting Extract Macerated with Aloe Vera Gel to Combat Mosquito Attacks

DYDIMUS EFEZE NKEMAJA, University of Bamenda, Bambili, Cameroon
SERGE ENAMA ETEME, University of Yaoundé 1, Laboratory of Parasitology and Ecology, Department of Animal Biology and Physiology, Faculty of Sciences, Cameroon
ABDOURAMANE NSANGOU, University of Douala, Mechanical Laboratory (LME), ENSET Douala, Cameroon, University of Douala, Mechanics Laboratory, Research Group on Innovative Materials (GRMI), Cameroon
FABIEN EBANDA BETENE, University of Douala, Mechanical Laboratory (LME), ENSET Douala, Cameroon, University of Douala, Mechanics Laboratory, Research Group on Innovative Materials (GRMI), Cameroon
DOINA SIBIESCU, “Gheorghe Asachi” Technical University of Iaşi, “Cristofor Simionescu” Faculty of Chemical Engineering and Environmental Protection, Iaşi, Romania, doina.sibiescu@academic.tuiasi.ro
PIERRE MARCEL ANICET NOAH, University of Douala, Mechanical Laboratory (LME), ENSET Douala, Cameroon, University of Douala, Mechanics Laboratory, Research Group on Innovative Materials (GRMI), Cameroon
ACHILLE DÉSIRÉ BETENÉ OMGBA, University of Douala, Mechanical Laboratory (LME), ENSET Douala, Cameroon, University of Douala, Mechanics Laboratory, Research Group on Innovative Materials (GRMI), Cameroon
VALERY HAMBATÉ GOMDJÉ, Departments of Textile and Leather Engineering, ENSPM, University of Maroua, Cameroon    

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DOI: 10.5281/zenodo.13837762
Abstract
The objective of this study is to identify the metabolites in the extract of Sida rhombifolia fibers treated with Aloe vera gel using colored reactions and thin-layer chromatography, following analytical techniques. The results show that the three samples fibers not treated with Aloe vera gel, fibers treated with Aloe vera gel, and washed treated fibers contain flavonoids in low quantities. The first two samples also present sterols and triterpenes in medium quantities, while the washed treated fibers contain these in low quantities. Based on the chromatographic profiles, it can be concluded that the untreated fibers, treated fibers, and washed treated fibers extracts have nearly identical constituents, as the Rf values of the different spots are the same. The UV light absorption profile indicates the presence of chromophores responsible for this absorption, specifically double and triple bonds, which are also confirmed by fluorescence (π-π* transition). The different colors observed include yellow for flavonoids, blue for triterpenes, black for alkaloids, and orange for sterols. Looking ahead, the experiments recommended by the World Health Organization (WHO) on the effectiveness of impregnated curtains in combating mosquito attacks (cone test, cylinder test, and tunnel test) will be the focus of future work.
Keywords: Knitting, phytochemical, screening, Sida rhombifolia